50-81-7Relevant articles and documents
Strategy for in Situ Imaging of Cellular Alkaline Phosphatase Activity Using Gold Nanoflower Probe and Localized Surface Plasmon Resonance Technique
Wang, Kan,Jiang, Ling,Zhang, Fen,Wei, Yuanqing,Wang, Kang,Wang, Huaisheng,Qi, Zhengjian,Liu, Songqin
, p. 14056 - 14062 (2018)
In this work, a simple and ultrasensitive localized surface plasmon resonance (LSPR) method that use Au nanoflowers (AuNFs) as a probe was designed for in situ monitoring of alkaline phosphatase (ALP) activity. The AuNFs were fabricated by hydrogen tetrechloroaurate-induced oxidative disruption of polydopamine-coated Au nanoparticles (AuNPs), and subsequently, growth of Au nanopetals on AuNPs occurred. The as-prepared AuNFs showed a much higher LSPR capability and stronger scattering color change than AuNPs. The strategy for in situ cellular ALP activity detection relied on the deposition of Ag on the AuNFs surface, which changed the morphology of AuNFs and led to a tremendous LSPR response and scattering color change. The deposition of Ag shell on AuNFs was related to ALP activity, where ALP catalyzed the hydrolysis of l-ascorbic acid 2-phosphate sesquimagnesium salt hydrate to form l-ascorbic acid (AA), and then AA reduced Ag+ to Ag and deposited onto AuNFs. With this concept, the ALP activity could be monitored with a detection limit of 0.03 μU L-1. Meanwhile, the ALP activity of single HepG2 cells and HEK 293 cells was tracked with a proposed approach, which indicated the trace expression level of ALP in HEK 293T cell and overexpressed level of ALP in HepG2 cells. After treatment with drugs, the cellular ALP activity of HepG2 cells was decreased with the treating time and dose increasing. Therefore, the proposed strategy could be used for tracking the cellular ALP activity, which paved a new avenue for cell studies and held great potential for discovering novel ALP-based drugs applications.
In Situ Fluorogenic Reaction Generated via Ascorbic Acid for the Construction of Universal Sensing Platform
Fan, Yongchao,Lv, Mengmeng,Xue, Yuan,Li, Jing,Wang, Erkang
, p. 6873 - 6880 (2021)
A highly fluorescent emission reaction between terephthalic acid (PTA) and ascorbic acid (AA) via simple control of the reaction temperature was first revealed with the detailed formation mechanism and various characterizations including electron paramagnetic resonance and mass spectrometry. Based on the AA-responsive emission, the alkaline phosphatase (ALP) triggered the transformation of l-ascorbic acid 2-phosphate trisodium salt to AA was integrated with the present system for developing a sensitive, selective, and universal platform. The monitoring of the activity of ALP and the fabrication of ALP-based enzyme-linked immunoassay (ELISA) with carcinoembryonic antigen (CEA) as the model target was performed. The fluorescence intensity correlated well to the CEA concentration in the ranges of 0.25-30 ng/mL, with a detection limit of 0.08 ng/mL. Such a facile protocol based on the fluorescent reaction between PTA and AA without the assistance of catalysis of nanomaterials avoided the laborious synthesis procedure and provided a direct strategy for the early clinical diagnosis coupled with ALP-related catalysis.
An efficient three step synthesis of vitamin C from L-galactono-1,4-lactone, a by-product of the sugar industry
Csiba,Cleophax,Petit,Gero
, p. 5059 - 5060 (1992)
An efficient and short synthesis of vitamin C has been accomplished from L-galactono-1,4-lactone via methyl 3,5:4,6-di-O-ethylidine-L-galactonate.
Redox turnover of organometallic B12 cofactors recycles vitamin C: Sulfur assisted reduction of dehydroascorbic acid by cob(II)alamin
Dereven'kov, Ilia A.,Hannibal, Luciana,Dürr, Maximilian,Salnikov, Denis S.,Bui Thi, Thu Thuy,Makarov, Sergei V.,Koifman, Oscar I.,Ivanovi?-Burmazovi?, Ivana
, p. 53 - 59 (2017)
This work reports the reactivity of cob(II)alamin (Cbl(II)) toward reduction of dehydroascorbic (DHA) to ascorbic acid (AA) mediated by sulfur-containing compounds such as glutathione (GSH) and thiocyanate. The reaction supported by GSH proceeded more efficiently than with SCN?. Our findings demonstrate new aspects of interactions between vitamins B12 and C. It has been accepted that simultaneous presence of these vitamins results in their decomposition (viz., irreversible modification of the corrin ring of Cbl and oxidation of AA). We have shown, however, that Cbl(II), the biologically active one-electron reduction product of methyl-Cbl (MeCbl) and adenosyl-Cbl (AdoCbl), is capable of recovering AA in the presence of natural sulfur-containing ligands, within a process that can occur in?vivo without glutathione spending, both in a stoichiometric and catalytic manner. Our studies highlight the redox versatility of Cbl(II) and expands the repertoire of reactions whereby redox turnover of the unique B12 organometallic cofactors MeCbl and AdoCbl generates Cbl(II), which in turn recycles oxidized vitamin C.
Interconversion between dehydro-L-ascorbic acid and L-ascorbic acid.
Nishikawa,Kurata
, p. 476 - 483 (2000)
L-Ascorbic acid (AA) plays an important role in biological systems as an electron donor. Erythorbic acid (EA) is the epimer of AA and has chemical characteristics very similar to those of AA. It is demonstrated in the present study by 1H-NMR that dehydro-L-ascorbic acid (DAA) was reduced by EA under neutral conditions but not acidic, and that dehydroerythorbic acid (DEA) was also reduced by AA under the same conditions. These reactions also occurred at a low concentration close to the concentration of AA in such biological tissue as the liver. Furthermore, the interconversion of DAA and AA at neutral pH and low concentration was also confirmed by radioluminography. These results suggest the interconversion between DAA and AA in vivo.
Fluorescence Immunoassay Based on the Alkaline Phosphatase Triggered in Situ Fluorogenic Reaction of o-Phenylenediamine and Ascorbic Acid
Zhao, Dan,Li, Juan,Peng, Chuanyun,Zhu, Shuyun,Sun, Jian,Yang, Xiurong
, (2019)
Inspired by the special reducing capability of ascorbic acid (AA), ascorbic acid 2-phosphate (AA2P) has been extensively utilized as a substrate in current alkaline phosphatase (ALP) activity assays owing to the ALP-triggered transformation of AA2P into AA. However, such assays usually require AA-related complicated and laborious synthesis and/or signal generation procedures. Herein, we report an interesting in situ fluorogenic interaction between o-phenylenediamine (OPD) and AA, which inspires us to put forward a novel and simple AA2P/OPD-participated fluorescence turn-on ALP activity assay for the first time, and then the corresponding ALP-based fluorescence enzyme-linked immunosorbent assay (ELISA) has also been developed by means of the conventional ELISA platforms. According to the convenient and facile detection process with clear response mechanism, our fluorogenic reaction-based assay exhibits good sensitivity, selectivity, and excellent sensing performance, which ensures fluorescence ELISA to potentially be applied in clinical diagnosis by employing a well-studied biomarker of hepatocellular carcinoma, α-fetoprotein (AFP) as the model analyte. Such original ELISA via in situ formation of fluorophore from scratch gives a new sight to develop other potential immunoassay platforms in early clinical diagnosis by controlling the target antigens in the near future.
A multicolor immunosensor for sensitive visual detection of breast cancer biomarker based on sensitive nadh-ascorbic-acid-mediated growth of gold nanobipyramids
Wang, Zongwen,Chen, Qian,Zhong, Yingying,Yu, Xinhui,Wu, Yongning,Fu, FengFu
, p. 1534 - 1540 (2020)
Many studies have demonstrated that the extracellular domain of human epidermal growth factor receptor 2 (HER2 ECD) level in serum can act as a breast cancer biomarker and serve as a monitoring neoadjuvant therapy of breast cancer. In this study, we developed a sensitive ascorbic acid (AA)-mediated AuNBPs (gold nanobipyramids) growth method with NADH (reduced nicotinamide adenine dinucleotide I) assistance, and we further fabricated a high-resolution multicolor immunosensor for sensitive visual detection of HER2 ECD in serum by using AuNBPs as signal and antibody as recognition probe. The NADH-assisted AA-mediated method effectively suppressed color formation in the blank and greatly improved the sensitivity of mediating AuNBPs growth, allowing us to use a low concentration of AA to mediate AuNBPs growth to generate more colorful and clearer color changes. The proposed multicolor immunosensor has higher resolution and more color changes corresponding to HER2 ECD concentrations. It can be used to detect as low as 0.5 ng/mL of HER2 ECD by bare eye observation and 0.05 ng/mL of HER2 ECD by UV-visible spectrophotometry. Using the immunosensor, we have successfully detected HER2 ECD in human serum with a recovery of 94%-96% and an RSD (n = 5) 5%. The results obtained with our immunosensor were consistent with those obtained with ELISA, verifying the immunosensor has good accuracy. The immunosensor exhibited a vivid multicolor change, has low visual detection limit, excellent specificity and reproducibility, and robust resistance to matrix. All the above features makes our immunosensor a promising assay for the early diagnosis of HER2-dependent breast cancers in clinical diagnosis.
In Situ Exsolution of Noble-Metal Nanoparticles on Perovskites as Enhanced Peroxidase Mimics for Bioanalysis
Jiang, Xiaoqian,Wang, Xiaoyu,Lin, Anqi,Wei, Hui
, p. 5954 - 5962 (2021)
Various transition-metal oxide (TMO)-based nanomaterials have been explored as peroxidase mimics. However, the moderate peroxidase-like activity of TMOs limited their widespread use. Decorating highly active noble-metal nanozymes on the surface of TMOs can not only enhance the peroxidase-like activity of TMOs but also prevent the small-sized metal nanoparticles (NPs) from aggregation. Herein, in situ exsolution of noble-metal NPs (i.e., Ir and Ru) from A-site-deficient perovskite oxides (i.e., chemical formula La0.9B0.9B'0.1O3-d, B = Mn/Fe, B' = Ir/Ru) under a reducing atmosphere was achieved for preparing noble-metal NPs/perovskite composites. The exsolved NPs were socketed on the surface of parent perovskite oxides, which significantly enhanced the stability of metal NPs. In addition, the peroxidase-like activity of perovskite oxides increased remarkably after NPs egress. We then used the optimized Ir/LMIO with high stability and excellent peroxidase-like activity to develop a colorimetric assay for the determination of alkaline phosphatase (ALP). Benefiting from the remarkable peroxidase-like activity of Ir/LMIO, the sensing platform exhibited a wide linear range. The practical application of the colorimetric sensing method was demonstrated by detecting the ALP in serum samples. This work not only provides new insights into the synthesis of highly active peroxidase-like nanozymes but expands their applications for constructing a high-performance biosensing platform.
Phosphate-triggered ratiometric fluoroimmunoassay based on nanobody-alkaline phosphatase fusion for sensitive detection of 1-naphthol for the exposure assessment of pesticide carbaryl
Chen, Zi-Jian,Wu, Hui-Ling,Shen, Yu-Dong,Wang, Hong,Zhang, Yi-Feng,Hammock, Bruce,Li, Zhen-Feng,Luo, Lin,Lei, Hong-Tao,Xu, Zhen-Lin
, (2021/10/12)
The excessive use of carbaryl has resulted in the risk of its exposure. In this study, we isolated six nanobodies (Nbs) from a camelid phage display library against the biomarker of carbaryl, 1-naphthol (1-NAP). Owing to its characteristics of easy genetic modifications, we produced a nanobody-alkaline phosphatase (Nb-CC4-ALP) fusion protein with good stability. A dual-emission system based ratiometric fluoroimmunoassay (RFIA) for quick and highly sensitive determination of 1-NAP was developed. Silicon nanoparticles (SiNPs) was used as an internal reference and for aggregation-induced emission enhancement (AIEE) of gold nanoclusters (AuNCs), while AuNCs could be quenched by MnO2 via oxidation. In the presence of ALP, ascorbic acid phosphate (AAP) can be transformed into ascorbic acid (AA), the later can etch MnO2 to recover the fluorescence of the AuNCs. Based on optimal conditions, the proposed assay showed 220-fold sensitivity improvement in comparison with conventional monoclonal antibody-based ELISA. The recovery test of urine samples and the validation by standard HPLC-FLD demonstrated the proposed assay was an ideal tool for screening 1-NAP and provided technical support for the monitoring of carbaryl exposure.
C a process for the preparation of vitamin
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Paragraph 0049-0052, (2018/02/04)
The invention relates to a preparation method of vitamin C. The preparation method comprises the following steps: by using gulonic acid inorganic salt or gulonic acid as the raw material, adding a 0-38 wt% hydrochloric acid solution, stirring, cooling, introducing HCl gas, heating, and keeping the temperature until the reaction finishes, thereby obtaining the vitamin C. The preparation method has the advantages of simple technique, short reaction time and more environment-friendly production environment, obviously enhances the vitamin C quality and is suitable for vigorous popularization.
